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Registros recuperados : 13 | |
3. | | NARANCIO, R.; JOHN, U.; MASON, J.; GIRALDO, P.; SPANGENBERG, G. Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens). Molecular Biology Reports, 2021, volume 48, Issue 4, Pages 3069 - 3077. Doi: https://doi.org/10.1007/s11033-021-06354-5 Article history: Received, 03 February 2021; Accepted, 12 April 2021; Published, 16 April 2021.
This project was funded by Agriculture Victoria, and Dairy Australia.Biblioteca(s): INIA Las Brujas. |
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4. | | NARANCIO, R.; JOHN, U.; MASON, J.; SPANGENBERG, G. Selection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.). Functional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304 Article history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018.
Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne,...Biblioteca(s): INIA Las Brujas. |
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5. | | DALLA RIZZA, M.; DIAZ, P.; NARANCIO, R.; CABRERA, A.; FERREIRA, F. Biomolecules as host defense weapons against microbial pathogens. (Review) Recent Patents on DNA and Gene Sequences, 2008, v.2, no.2, p.82-96. Article history: Received: April 21, 2008 / Accepted: April 29, 2008 / Revised: April 29, 2008.Biblioteca(s): INIA Las Brujas. |
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7. | | ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. Article history: Received September 7 2010. /Accepted June 21, 2011.Biblioteca(s): INIA La Estanzuela. |
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8. | | NARANCIO, R.; ZORRILLA, P.; ROBELLO, C.; GONZÁLEZ-ARCOS, M.; VILARÓ, F.; PRITSCH, C.; DALLA RIZZA, M. Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum. European Journal of Plant Pathology, 2013, v.136, no.4, p.823-835. Article history: Accepted: 27 March 2013 / Published online: 28 April 2013.Biblioteca(s): INIA Las Brujas. |
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9. | | REAL, D.; REYNO, R.; DALLA RIZZA, M.; ALTIER, N.; DO CANTO, J.; NARANCIO, R.; FOLLE, G.; BURGUEÑO, J. Caracterización de genotipos de Paspalum notatum por tolerancia a bajas temperaturas y reacción frente a Claviceps paspali ln: Reunión del grupo técnico en forrajeras del Cono Sur, 22., 2008, Minas, Uruguay Bioma campos: innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): INIA; FAO; PROCISUR, 2008. p. 156 Versión impresa y en CD ROM Instituto Nacional de Investigación Agropecuaria, Uruguay; FAO; PROCISURBiblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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10. | | NARANCIO, R.; DING, Y.L.; LIN, Y.H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; MASON, J.; SPANGENBERG, G. Generación y caracterización molecular y funcional de plantas de trébol blanco transgénicas con las características de senescencia retardada, tolerancia a aluminio y resistencia a Alfalfa Mosaic Virus (AMV). [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p.10-11. (Serie Actividades de Difusión; 786)Biblioteca(s): INIA Las Brujas. |
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11. | | NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. Application of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.). Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 Article history: Received: 19 May 2020 / Accepted: 15 July 2020.Biblioteca(s): INIA Las Brujas. |
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12. | | NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 Article history: Received: 19 May 2020 / Accepted: 15 July 2020.Biblioteca(s): INIA Treinta y Tres. |
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13. | | REYNO, R.; NARANCIO, R.; SPERANZA, P.; DO CANTO, J.; LOPEZ-CARRO, B.; HERNANDEZ, P.; BURGUEÑO, J.; REAL, D.; DALLA RIZZA, M. Molecular and cytogenetic characterization of a collection of bahiagrass (Paspalum notatum Flügge) native to Uruguay. Genetic Resources and Crop Evolution, 2012, v. 59, no.8, p. 1823-1832. Article history: Received: 9 August 2011 / Accepted: 30 January 2012 / Published online: 22 February 2012.Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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Registros recuperados : 13 | |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
11/03/2021 |
Actualizado : |
11/03/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
NARANCIO, R.; JOHN, U.; MASON, J.; SPANGENBERG, G. |
Afiliación : |
RAFAEL NARANCIO FERES, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia. |
Título : |
Selection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.). |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Functional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304 |
ISSN : |
1445-4408 |
DOI : |
10.1071/FP17304 |
Idioma : |
Inglés |
Notas : |
Article history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018.
Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia; email:ulrik.john@ecodev.vic.gov.au |
Contenido : |
ABSTRACT -
Quantitative reverse transcription PCR (qRT-PCR) is a widely used method for transcript abundance analyses in plants. Relative quantification by qRT-PCR requires the use of a stably expressed reference gene. There are many ?housekeeping? genes reported in different plant species that are used as reference genes. However, it is important that the steady-state mRNA levels of these housekeeping genes are confirmed across different conditions and tissues in each species studied. Prior to this study, no comprehensive work had been performed in identifying optimal reference genes in white clover (Trifolium repens L.). To identify suitable reference genes in white clover, we analysed the transcript abundance stability of seven candidate genes in two organs (leaves and stolons) across two treatments (water-limited and well-watered). DCt, NormFinder and ANOVA tests were carried out to evaluate the mRNA level stability of candidate reference genes. According to the DCt results, the genes with the most stable mRNA levels were EF1a and ACT11. When stability among groups was evaluated by NormFinder, UBQ was the most stable across all organs and treatments.
By multiple criteria, EF1a, followed by ACT11 and UBQ, was the most stably-expressed gene across organs and treatments, and each of these are recommended as reference genes for transcript abundance studies in white clover.
© Copyright 2018 Elsevier B.V., All rights reserved. |
Palabras claves : |
ACt; Gene Selection; Housekeeping genes; MRNA level stability; NormFinder; Peptide Elongation Factor 1. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02570naa a2200265 a 4500 001 1061825 005 2021-03-11 008 2018 bl uuuu u00u1 u #d 022 $a1445-4408 024 7 $a10.1071/FP17304$2DOI 100 1 $aNARANCIO, R. 245 $aSelection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.).$h[electronic resource] 260 $c2018 500 $aArticle history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018. Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia; email:ulrik.john@ecodev.vic.gov.au 520 $aABSTRACT - Quantitative reverse transcription PCR (qRT-PCR) is a widely used method for transcript abundance analyses in plants. Relative quantification by qRT-PCR requires the use of a stably expressed reference gene. There are many ?housekeeping? genes reported in different plant species that are used as reference genes. However, it is important that the steady-state mRNA levels of these housekeeping genes are confirmed across different conditions and tissues in each species studied. Prior to this study, no comprehensive work had been performed in identifying optimal reference genes in white clover (Trifolium repens L.). To identify suitable reference genes in white clover, we analysed the transcript abundance stability of seven candidate genes in two organs (leaves and stolons) across two treatments (water-limited and well-watered). DCt, NormFinder and ANOVA tests were carried out to evaluate the mRNA level stability of candidate reference genes. According to the DCt results, the genes with the most stable mRNA levels were EF1a and ACT11. When stability among groups was evaluated by NormFinder, UBQ was the most stable across all organs and treatments. By multiple criteria, EF1a, followed by ACT11 and UBQ, was the most stably-expressed gene across organs and treatments, and each of these are recommended as reference genes for transcript abundance studies in white clover. © Copyright 2018 Elsevier B.V., All rights reserved. 653 $aACt 653 $aGene Selection 653 $aHousekeeping genes 653 $aMRNA level stability 653 $aNormFinder 653 $aPeptide Elongation Factor 1 700 1 $aJOHN, U. 700 1 $aMASON, J. 700 1 $aSPANGENBERG, G. 773 $tFunctional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304
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